Review



pspcas9 bb 2a puro plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Addgene inc pspcas9 bb 2a puro plasmid
    Pspcas9 Bb 2a Puro Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm42848794-247-11-13
    Average 96 stars, based on 2950 article reviews
    pspcas9 bb 2a puro plasmid - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: MuSK is a substrate for CaMK2β but this interaction is dispensable for MuSK activation in vivo
    Article Snippet: .. Oligonucleotides encoding Cas9-target sequences on exon 2 or exon 8 of the murine Camk2b gene were obtained from Microsynth (Balgach, Switzerland) and cloned into pX459 v2.0 (pSpCas9(BB)−2A-Puro, Addgene plasmid #62988 ), using Bpil. ..

    Article Title: Lamin A/C-regulated cysteine catabolic flux modulates stem cell fate through epigenome reprogramming
    Article Snippet: .. Annealed gRNAs were cloned into PX459 V2.0 (Addgene #62988) and transfected using Lipofectamine 2000. ..

    Article Title: Lamin A/C-regulated cysteine catabolic flux modulates stem cell fate through epigenome reprogramming.
    Article Snippet: .. Annealed gRNAs were cloned into PX459 V2.0 (Addgene #62988) and transfected using Lipofectamine 2000. ..

    Plasmid Preparation:

    Article Title: MuSK is a substrate for CaMK2β but this interaction is dispensable for MuSK activation in vivo
    Article Snippet: .. Oligonucleotides encoding Cas9-target sequences on exon 2 or exon 8 of the murine Camk2b gene were obtained from Microsynth (Balgach, Switzerland) and cloned into pX459 v2.0 (pSpCas9(BB)−2A-Puro, Addgene plasmid #62988 ), using Bpil. ..

    Article Title: Quantification profiles of enzyme activity, secretion, and psychosine levels of Krabbe disease galactosylceramidase missense variants
    Article Snippet: .. Guided RNA targeting sequence 1 (sgRNA-GALC1: 5′ GAAAGCTATGACTGCGGCCG 3′) and 2 (sgRNA-GALC2: 5′ TACGTGCTCGACGACTCCGA 3′) were subcloned individually into pX459 v2.0 (gift from Dr Feng Zhang, Addgene plasmid #62988 ( )). ..

    Article Title: Prediction of the hypothalamus-pituitary organoid formation using machine learning
    Article Snippet: .. pX459 v2.0 , Addgene plasmid , #62988. ..

    Amplification:

    Article Title: Engineered AcrIIA5 for optogenetic control of CRISPR‐Cas9‐based genome editing
    Article Snippet: Site‐directed mutagenesis of AsLOV2 was performed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme). .. The DNA fragments of SpCas9 , SaCas9 , NmeCas9 , and St1Cas9 were amplified from Px459 V2.0 (Addgene #62988), BPK2101 (Addgene #65770), pEJS654 (Addgene #112139), and MSP1673 (Addgene #65769), respectively. .. These fragments were cloned into the backbone of pCMV‐BE3 (Addgene #73021) to construct pCMV‐SpCas9, pCMV‐SaCas9, pCMV‐NmeCas9, and pCMV‐St1Cas9.

    Article Title: Engineered AcrIIA5 for optogenetic control of CRISPR‐Cas9‐based genome editing
    Article Snippet: Site‐directed mutagenesis of AsLOV2 was performed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme). .. The DNA fragments of SpCas9, SaCas9, NmeCas9, and St1Cas9 were amplified from Px459 V2.0 (Addgene #62988), BPK2101 (Addgene #65770), pEJS654 (Addgene #112139), and MSP1673 (Addgene #65769), respectively. .. These fragments were cloned into the backbone of pCMV‐BE3 (Addgene #73021) to construct pCMV‐SpCas9, pCMV‐ SaCas9, pCMV‐NmeCas9, and pCMV‐St1Cas9.

    Sequencing:

    Article Title: Quantification profiles of enzyme activity, secretion, and psychosine levels of Krabbe disease galactosylceramidase missense variants
    Article Snippet: .. Guided RNA targeting sequence 1 (sgRNA-GALC1: 5′ GAAAGCTATGACTGCGGCCG 3′) and 2 (sgRNA-GALC2: 5′ TACGTGCTCGACGACTCCGA 3′) were subcloned individually into pX459 v2.0 (gift from Dr Feng Zhang, Addgene plasmid #62988 ( )). ..

    Transfection:

    Article Title: Lamin A/C-regulated cysteine catabolic flux modulates stem cell fate through epigenome reprogramming
    Article Snippet: .. Annealed gRNAs were cloned into PX459 V2.0 (Addgene #62988) and transfected using Lipofectamine 2000. ..

    Article Title: Lamin A/C-regulated cysteine catabolic flux modulates stem cell fate through epigenome reprogramming.
    Article Snippet: .. Annealed gRNAs were cloned into PX459 V2.0 (Addgene #62988) and transfected using Lipofectamine 2000. ..



    Similar Products

    96
    Addgene inc pspcas9 bb 2a puro plasmid
    Pspcas9 Bb 2a Puro Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm42848794-247-11-13
    Average 96 stars, based on 1 article reviews
    pspcas9 bb 2a puro plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc pspcas9 bb 2a puro
    Pspcas9 Bb 2a Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm42844252-380-19-8
    Average 96 stars, based on 1 article reviews
    pspcas9 bb 2a puro - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc px459 v2 plasmid
    Px459 V2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/10__1038_slash_s41467___026___77999___8-444-6-9
    Average 96 stars, based on 1 article reviews
    px459 v2 plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc plasmid pspcas9 bb 2a puro px459 v2 0
    Plasmid Pspcas9 Bb 2a Puro Px459 V2 0, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm42827260-438-5-7
    Average 96 stars, based on 1 article reviews
    plasmid pspcas9 bb 2a puro px459 v2 0 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc px459

    Px459, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pmc13672076-38-0-2
    Average 96 stars, based on 1 article reviews
    px459 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc pcas9 expression vector

    Pcas9 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/10__28978_slash_nesciences__263004-47-1-4
    Average 96 stars, based on 1 article reviews
    pcas9 expression vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc plasmid pspcas9 bb 2a puro v2 0

    Plasmid Pspcas9 Bb 2a Puro V2 0, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/10__1038_slash_s41419___026___09199___7-405-7-16
    Average 96 stars, based on 1 article reviews
    plasmid pspcas9 bb 2a puro v2 0 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc px459 plasmid

    Px459 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/10__1038_slash_s41598___026___63336___y-49-18-22
    Average 96 stars, based on 1 article reviews
    px459 plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc feng zhang

    Feng Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+v2+0/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm41920822-295-17-20
    Average 96 stars, based on 1 article reviews
    feng zhang - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Journal: PNAS Nexus

    Article Title: Identifying common G protein–coupled receptor interacting proteins independent of heterotrimeric G proteins and β-arrestins

    doi: 10.1093/pnasnexus/pgag327

    Figure Lengend Snippet:

    Article Snippet: PX459 , Addgene , Cat#62988; RRID:Addgene_62988.

    Techniques: Recombinant, Protease Inhibitor, Titration, Mutagenesis, Mass Spectrometry, Knock-Out, Software